單克隆抗體的生產(chǎn)，1999年出版,共計60余頁，GIF格式。
CONTENTS
COMMITTEE ON METHODS OF PRODUCING MONOCLONAL ANTIBODIES iii
PREFACEVII
CONTENTSIX
EXECUTIVE SUMMARY 1
INTRODUCTION 4
GENERATION OF HYBRIDOMAS: PERMANENT CELL LINES SECRETING MONOCLONAL
ANTIBODIES 6
IN VITRO PRODUCTION OF MONOCLONAL ANTIBODY 9
BATCH TISSUE-CULTURE METHODS 9
SEMIPERMEABLE-MEMBRANE-BASED SYSTEMS 10
SCIENTIFIC NEEDS FOR MOUSE ASCITES PRODUCTION OF MAB 12
SUMMARY OF ADVANTAGES AND DISADVANTAGES OF IN VITRO AND IN VIVO
METHODS 16
ADVANTAGES OF IN VITRO METHODS 16
DISADVANTAGES OF IN VITRO METHODS 16
ADVANTAGES OF MOUSE ASCITES METHOD 17
DISADVANTAGES OF MOUSE ASCITES METHODS 17
LARGE-SCALE PRODUCTION OF MONOCLONAL ANTIBODIES 18
MONOCLONAL ANTIBODY PRODUCTION FOR DIAGNOSTIC AND THERAPEUTIC PURPOSES 18
IN VIVO AND IN VITRO METHODS FOR COMMERCIAL PRODUCTION OF MAB 19
In Vivo Production 20
In Vitro Production 21
REGULATORY REQUIREMENTS 23
ANIMAL-WELFARE ISSUES RELATED TO THE ASCITES METHOD FOR PRODUCING
MONOCLONAL ANTIBODIES 24
AVAILABILITY OF DATA 24
ANIMAL-WELFARE ISSUES RELATED TO ASCITES METHOD 24
METHODS FOR MEASURING PAIN OR DISTRESS IN LABORATORY RODENTS 25
PRIMING 26
ASCITES 27
HARVESTING ASCITIC FLUID 29
FEEDER CELL HARVESTING AND SERUM SUPPLEMENTS FOR IN VITRO HYBRIDOMA CULTURE 30
SUMMARY OF ANIMAL WELFARE ISSUES 30
CONCLUSIONS AND RECOMMENDATIONS 31
ix
REFERENCES 34
APPENDIX A 44
WORKSHOP ON METHODS OF PRODUCING MONOCLONAL ANTIBODIES 44
APPENDIX B 46
BIOGRAPHICAL SKETCHES OF AUTHORING COMMITTEE 46
INDEX
