The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.
分成五個不同的程序,分別為:blastp(提交蛋白質(zhì)序列,在蛋白質(zhì)序列數(shù)據(jù)庫中查序列)、blastn(提交核酸序列,在核酸序列數(shù)據(jù)庫中查找同源序列) blastx(提交核酸序列,在蛋白質(zhì)序列數(shù)據(jù)庫中查找同源序列)、tblastn (提交蛋白質(zhì)序列,在核酸序列數(shù)據(jù)庫中查找同源序列)、tblastx(提交核酸序列,在核酸序列數(shù)據(jù)庫中查找同源序列)。通常通過在線方式或Email方式提交查詢序列,得到查詢結(jié)果。如果需要在本地使用的話,可以下載BLAST程序與相應(yīng)數(shù)據(jù)庫,便能在本地使用了。DNA TOOL可以調(diào)用此軟件,參見DNA分析類。